FastQCFastQC Report
Sun 14 Apr 2019
SRR6575849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516374
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA48570.3203035662705902No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC47990.31647865236412653No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC40390.2663590908311538No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG23270.15345818379898363No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT22380.14758891935630655No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19030.1254967442069041No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18730.12351834046218149No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT18730.12351834046218149No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT17140.11303280061515167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28750.047.58021
GGTATCA10300.042.9004441
TCAACGC31850.042.497984
ATCAACG32350.041.695853
CAACGCA32750.041.186595
AACGCAG34400.039.0744446
GGTAATC4700.034.998988
TATCAAC38450.034.9655882
ACGCAGA42150.031.8899367
AGGTATA13300.031.8035876
CTTAGGT13200.031.6884733
GTACATG62400.031.4892881
TTAGGTA13400.031.2155114
TAGGTAT13700.030.8750135
ACATGGG65900.029.739653
TACATGG65350.029.708152
GTCTTAG15200.029.689221
TCTTAGG15650.028.8355352
ACCTAAG19600.028.7803671
GGTATAG14600.028.649857