FastQCFastQC Report
Sun 14 Apr 2019
SRR6575849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516374
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52830.3483968994456513No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC50080.33026153178569406No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC41300.27236024885681237No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG23800.15695336374799357No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT22590.1489738019776124No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT19980.1317616893985257No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT17870.11784691639397668No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17170.11323064098962393No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16530.10901004633421571No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15830.10439377092986296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26600.051.372111
TCAACGC30900.043.1983034
CAACGCA31450.042.590895
ATCAACG31350.042.5810433
GGTATCA9550.041.4464071
AACGCAG32550.041.1515666
GGTAATC4400.037.3859028
TATCAAC38500.034.5510442
ACGCAGA40850.032.7902957
GTCTTAG15150.031.4137461
GTACATG59600.031.1502081
TTAGGTA14100.031.0006434
AGGTATA13750.030.4214446
TACATGG61050.030.3351442
TAGGTAT13950.030.3222085
ACCAGAT19250.030.27495694
CTTAGGT14050.030.109373
CGCAGAG45750.029.5865218
ACCTAAG20800.028.5442241
ACATGGG65050.028.3975433