FastQCFastQC Report
Sun 14 Apr 2019
SRR6575850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372287
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA48460.3531331273997349No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC44960.32762825852026584No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC39780.28988105257865154No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG21940.15987909234730052No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT21440.1562355396502335No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18400.13408273925206607No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT17510.1275972154512868No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17340.126358407534284No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT17160.12504672856333987No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15480.11280439150119473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.047.0038641
GTATCAA24350.046.7143141
TCAACGC28400.039.5430954
ATCAACG28850.038.9263083
CAACGCA29450.038.1332445
AACGCAG30650.036.6402556
TAGGTAT12550.033.3224835
AGGTATA12600.033.1902546
TATCAAC34500.033.1082762
TTAGGTA12600.032.817334
ACCAGAT16450.032.56342794
GTCTTAG14050.031.7819731
CTTAGGT13050.031.68573
GGTATAG13650.030.6371577
GTACATG58250.030.1793041
TACATGG59000.029.4780732
TATAGTA14550.029.0639639
TCTTAGG15050.029.0466352
ACGCAGA39550.028.6326567
GTATAGT14850.028.4778468