FastQCFastQC Report
Sun 14 Apr 2019
SRR6575850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372287
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA51230.37331840934148613No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC48040.3500725431341986No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC39510.28791353412223536No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG22280.1623567081813061No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT20730.15106169482039836No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT18140.13218809184959124No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17330.12628553648034266No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT16990.1238079206463371No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16090.11724952579161647No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14770.10763054667135956No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14690.10704757823982886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24200.049.0186041
GGTATCA9650.045.3660931
TCAACGC29800.038.1619384
ATCAACG29850.038.098023
CAACGCA31050.036.625635
AACGCAG32300.035.353726
TCTTAGG13750.032.4677542
GTCTTAG13150.032.217551
TATCAAC36050.031.9368572
CTTAGGT12600.031.7014923
AGGTATA13150.031.0902966
TAGGTAT13000.031.0875475
GTACATG58150.030.9235531
TTAGGTA12850.030.719034
GGTAATC3400.030.4281758
TACATGG60750.029.7040942
ACATGGG62050.028.93033
CATATAC3250.028.9186483
ATTATCC21200.028.5946773
CCCTATA1652.9704097E-928.4804882