FastQCFastQC Report
Sun 14 Apr 2019
SRR6575851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551808
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22160.14280117127892109No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20740.1336505547077989No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19740.1272064585309523No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19140.12334000082484431No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17730.11425382521549057No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16310.10510320864436837No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16180.10426547614137832No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15900.10246112921186126No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15760.10155895574710273No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15750.10149451478533426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25450.052.831161
TCAACGC31250.041.208474
ATCAACG31300.041.1426433
CAACGCA32100.040.117285
AACGCAG33150.038.9883736
GGTATCA10500.037.162141
TATCAAC39250.033.417692
TTAGGTA7050.033.332424
GTCTTAG9650.032.6406941
ACCTAAG9550.031.997931
CTTAGGT7550.031.7474733
ACGCAGA42650.030.3039747
CTAAGAC11550.030.1117273
GTACATG64350.029.6612451
TAAGACA12450.029.4449774
TACATGG65850.028.7714122
CCCTATA1652.9576768E-928.492332
TAGGTAT7550.028.0124765
ACATGGG67600.027.8098893
AGGTATA7850.027.5406466