FastQCFastQC Report
Sun 14 Apr 2019
SRR6575852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424668
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21510.15098254470515238No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20610.14466528342041796No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19430.13638265195821062No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18810.13203076085094914No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16730.1174308681040074No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15380.10795497617690578No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15220.10683190750406411No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14600.10248001639680263No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14390.10100598876369793No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14320.1005146462193297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.051.5267331
GGTATCA8300.042.4743961
TCAACGC28400.038.7145234
ATCAACG28650.038.37673
CAACGCA29250.037.5894855
AACGCAG30500.036.2029886
GTCTTAG8200.033.820671
TAGGTAT5700.032.9732325
CTTAGGT6250.032.3269583
TATCAAC35450.031.2913722
TTAGGTA6100.030.0407774
GTACATG56400.029.7531681
CCGCGTT650.006163071428.914999
TACATGG58400.028.4917582
AGGTATA6450.028.4106586
ACGCAGA38800.028.3374357
ACATGGG59800.027.5792433
CGCAGAG41600.026.6560068
TCTTAGG9900.026.1129742
GGTATAG7400.024.7633447