FastQCFastQC Report
Sun 14 Apr 2019
SRR6575852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424668
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23260.16326610831435814No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20010.14045377589726168No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18600.13055673321784445No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17070.11981738903379595No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14740.10346270148553907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21400.047.699921
GGTATCA10100.038.1912731
CTTAGGT7100.035.739593
TCAACGC28450.035.0160984
GTCTTAG9150.034.444941
TAGGTAT7250.034.3532075
ATCAACG29000.034.3523
AGGTATA7050.033.9946446
TTAGGTA7450.033.4298024
CAACGCA30450.033.334655
AACGCAG31250.032.6316576
GTACATG57700.031.0613861
GGTATAG8050.030.3554657
TACATGG59950.029.7073692
TTAGACG650.00616045228.9175034
ACATGGG61750.028.5370123
GTATAGT8450.028.3813198
TCTTAGG10550.028.0609442
TATCAAC36150.027.81762
TATAGTA8650.027.7251039
Produced by FastQC (version 0.11.2)