FastQCFastQC Report
Sun 14 Apr 2019
SRR6575855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795304
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11220.14107812861496988No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10770.13541991490046573No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9660.1214629877380222No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9650.12133724965547765No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8810.1107752507217366No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8620.1083862271533904No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8340.10486556084214338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.048.606951
GGTATCA4950.038.951021
TCAACGC15300.036.860134
ATCAACG15450.036.5022663
CAACGCA15800.035.6936725
AACGCAG16600.034.2566076
GTACATG29500.032.3604281
TACATGG30150.031.1948492
ACATGGG31350.030.2817443
GTCTTAG4100.029.8215141
AGGTATA3050.027.7357396
TATCAAC21200.027.505912
TACGGTC700.00883747226.8552385
TAGGTAT3350.026.6548275
ACGCAGA21600.026.3268387
CGCAGAG21850.025.8105288
CATGGGG18950.025.0483554
CTTAGGT3400.024.8805893
TATAAGC950.001299997324.7506482
GTATAGA1151.3714831E-424.5354251
Produced by FastQC (version 0.11.2)