FastQCFastQC Report
Sun 14 Apr 2019
SRR6575856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740024
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11730.1585083727014259No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10970.14823843551019966No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8900.12026636973935981No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8860.11972584672929527No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8330.11256391684594012No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7910.10688842524026249No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7750.10472633320000432No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7710.10418581018993979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.049.7896771
GGTATCA4600.040.9377021
TCAACGC13250.035.818254
GTACATG27850.035.32991
ATCAACG13550.035.0252273
ACATGGG28250.033.932093
CAACGCA14100.033.6589975
TACATGG29100.033.2661442
GTCTTAG3950.032.1801451
AACGCAG14950.032.059586
CTCGCGA300.004152250531.34737412-13
CTTAGGT3000.029.7598843
TATCAAC17450.027.737672
TAGGTAT3500.026.8510235
GGTACCT4950.026.5977738
CATGGGG17000.026.5351284
GGGTACC5150.026.4599887
CTATTAG909.4223884E-426.1546421
AGGTATA3450.025.878166
ACGCAGA18800.025.4941887
Produced by FastQC (version 0.11.2)