FastQCFastQC Report
Sun 14 Apr 2019
SRR6575857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734373
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20770.11975509305091811No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19580.11289382387756268No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19340.11151003849806242No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19330.11145238077391657No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18630.10741634008370748No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18630.10741634008370748No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18410.10614787015249892No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17960.10355327256593592No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17860.1029766953244775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24200.053.2264821
TCAACGC31400.040.2626654
ATCAACG31750.039.818823
CAACGCA33350.038.04945
AACGCAG34650.036.7574966
GGTATCA10000.033.847381
TATCAAC42200.030.4118442
TAGGTAT6700.028.7600045
GTACATG64950.028.7345181
GTCTTAG8600.028.4248051
CTAACGC1005.326575E-528.1988323
TACATGG65000.028.133832
ACGCAGA45200.027.8661047
GGTACCT9800.027.8151768
ACCTAAG7800.027.1212981
ACATGGG68300.027.0428033
CTTAGGT7000.026.856033
AGGTATA7050.026.6655626
TATACCG2650.026.602674
GGGTACC10300.026.0086337