FastQCFastQC Report
Sun 14 Apr 2019
SRR6575857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734373
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22790.13140195332837862No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19690.11352805884316695No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19440.11208661573952085No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18890.10891544091149942No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18440.10632084332493645No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18200.10493705794543619No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17430.1004974131862062No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17380.10020912456547698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23800.053.6791271
ATCAACG31700.039.2923553
TCAACGC31750.039.2304764
CAACGCA33550.037.265815
GGTATCA10450.036.541121
AACGCAG35400.035.583856
TTAGGTA5950.033.1782724
TAGGTAT5950.032.3883135
AGGTATA5850.031.3350336
GTACATG66100.030.8102631
TACATGG66050.030.5285322
GTCTTAG9600.029.9551641
ACATGGG67200.029.936153
TATCAAC42550.029.935822
CTTAGGT6750.029.2460333
ACGCAGA43500.028.9545547
GGTATAG6600.027.771037
CGCAGAG47850.026.5187598
GTATAGT7600.024.7353328
GGTACCT11100.024.557068