FastQCFastQC Report
Sun 14 Apr 2019
SRR6575858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561662
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20020.1281967544833645No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19420.12435469390943751No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18720.11987228990652267No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18330.11737495053347011No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18110.11596619498969687No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18100.11590216064679809No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17500.11206010007287108No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17300.11077941321489541No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17040.10911452029952705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21100.053.2419931
TCAACGC27500.040.4963074
ATCAACG27900.039.9157143
CAACGCA28100.039.4643945
AACGCAG29250.037.9128046
TTAGGTA6350.034.7795454
AGGTATA6650.034.6237566
TAGGTAT6800.033.8599975
GGTATCA9400.033.5031621
CTTAGGT7150.032.2025153
GTCTTAG8400.030.7767141
GTACATG57550.030.1383781
GGTATAG7500.030.0732067
ACGCAGA37500.029.5719877
TACATGG58200.029.3998452
ACCTAAG8100.029.0150851
TATAGTA8000.028.7791569
TATCAAC39400.028.633982
GTATAGT8150.028.2512868
ACATGGG60300.028.2092173