FastQCFastQC Report
Sun 14 Apr 2019
SRR6575858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561662
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22560.14446147757965552No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19400.12422662522363995No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18580.11897580910593969No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18200.11654250407578592No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17730.11353288995954311No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17650.11302061521635284No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17080.10937065767112218No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16510.10572070012589152No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16220.1038637041818268No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15680.1004058496652925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19250.054.773721
ATCAACG25950.038.9355473
TCAACGC26100.038.5317274
CAACGCA26750.037.5954445
GGTATCA7050.037.3898621
AACGCAG28650.035.10226
ACCTAAG7650.030.7654691
GTACATG55500.030.3630241
TACATGG55550.030.2861562
TAGGTAT6300.029.8376525
GTACTAA650.006109504528.9668731
GTCTTAG8850.028.721391
ACATGGG58150.028.608743
TATCAAC36950.028.3619172
ACGCAGA35600.028.1164767
AGGTATA6350.027.3825076
TAAGACA11850.027.363774
CTAAGAC11450.025.8571283
TTAGGTA6750.025.7598384
CTTAGGT7000.025.511193