FastQCFastQC Report
Sun 14 Apr 2019
SRR6575860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368479
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28300.20679893516816847No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23640.17274653100266793No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22680.1657314434492601No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16810.12283710601331843No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16660.12174099858309846No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16480.12042566966683449No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16100.11764886417694388No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16100.11764886417694388No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15950.11655275674672391No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15710.11479898485837196No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15070.11012225982276673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.051.8167651
GGTATCA9150.039.5605241
TCAACGC26700.038.0210234
AATCGCG500.001702459237.59856894
ATCAACG27450.036.810983
CAACGCA28400.035.9106065
TAGGTAT7650.035.632635
AGGTATA7700.035.401256
CTTAGGT7600.035.2486573
TTAGGTA7850.034.7247924
GTCTTAG9800.034.538131
GGTATAG8050.034.4458927
AACGCAG29900.034.1090666
CTAACGC600.00416203331.3321383
GTATAGT8800.030.4420228
TATAGTA8950.029.9318229
GTACATG56800.029.629691
TACATGG57500.029.1054672
TATCAAC35950.028.6376672
ACATGGG59450.028.6179183