FastQCFastQC Report
Sun 14 Apr 2019
SRR6575865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367942
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7360.20003152670801375No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5000.13589098281794415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG250.00524008256.3813259
GGTATCA1950.053.015291
GTATCAA6400.051.3962141
CAACGCA8400.040.831715
TCAACGC8700.040.503834
ATCAACG8450.040.034083
AACGCAG8750.038.6614846
AGCGTAC250.001704399437.5875582-83
TATCAAC9850.032.917432
TGTATCG600.00416408131.322969
GTACTAT753.277606E-431.322966
ATGGGAG5050.029.7723165
TTATATG804.7839058E-429.3652734
GTACATG14900.029.3298421
CTATACA650.00615875928.91354
ACGCAGA11600.028.7577177
ATTATCC3800.028.437953
TACATGG15650.027.924262
CGCAGAG12000.027.7991268
ACATGGG15900.027.4814663