FastQCFastQC Report
Sun 14 Apr 2019
SRR6575866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439372
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24250.1684762521432958No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19770.13735156721125602No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17290.12012183090959112No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16110.11192381121766992No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15350.10664373073812747No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15270.10608793279291247No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14910.10358684203944499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21100.054.5329061
GGTATCA7950.048.0473141
TCAACGC27800.040.580014
ATCAACG28150.039.9098663
CAACGCA29100.038.9286885
AACGCAG29900.037.7299086
TATCAAC36900.030.5735322
ACGCAGA37700.029.7938617
ACCTAAG9100.029.5382481
GTACATG53600.029.473471
TACATGG55600.028.5760822
ACATGGG56850.027.6997033
GTCTTAG8050.027.532981
CGCAGAG40700.027.4822838
CTAAGAC12200.027.3564223
TAAGACA13700.026.0758634
GCAGAGT45200.024.8501919
ATAGTAC1751.6444756E-724.1749323
AGGTATA5100.023.9634256
TTAGGTA5900.023.9009384
Produced by FastQC (version 0.11.2)