FastQCFastQC Report
Sun 14 Apr 2019
SRR6575867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329353
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21760.16368865154703077No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18550.13954156646127852No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16310.12269126409614302No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15690.1180273411200787No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14910.1121598251179333No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14840.11163325316902284No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14830.11155802860489275No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13820.10396034762775576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.054.9128651
TCAACGC25350.040.595284
GGTATCA6950.040.573381
ATCAACG25450.040.435773
CAACGCA26250.039.3824545
AACGCAG27950.037.3233456
TATCAAC33450.031.8948692
GTACATG52900.031.1835941
ACCTAAG8600.031.1495041
TACATGG53650.030.486012
ACGCAGA34100.030.4530627
ACATGGG55150.029.5660533
CTAAGAC11400.028.0293773
TAAGACA11800.027.8756794
CGCAGAG37550.027.7802458
CATGGGG32950.026.0978684
TAGGTAT6000.025.8447345
GTCTTAG8200.025.791311
GCAGAGT41400.025.3093629
CTTAGGT6000.025.0615623
Produced by FastQC (version 0.11.2)