FastQCFastQC Report
Sun 14 Apr 2019
SRR6575868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087722
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20250.1861688924192027No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17390.15987540934172517No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14210.13063999808774668No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13050.11997550844793062No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12690.11666583924936702No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12490.1148271341390539No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12000.11032230661878678No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11980.11013843610775548No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11120.10223200413340908No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11030.10140458683376818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30150.043.4997061
AGGTATA4500.036.5548556
CAACGCA36150.036.0131455
ATCAACG36600.035.570363
TCAACGC36500.035.5390474
TAGGTAT4700.034.9993325
CTTAGGT4850.034.8859333
AACGCAG37750.034.362266
GGTATAG5100.033.175847
TTAGGTA5000.032.8993724
GTATAGT5250.031.3327338
TATCAAC42200.031.1899762
TCTAACG1102.9644416E-629.914022
CTAACGC1102.9686944E-629.908523
GTACATG60500.029.5255281
CGTTAAC804.776106E-429.379841
GTCTTAG6900.029.2946851
ACGCAGA44400.029.1098047
TACATGG62750.028.5417542
ACATGGG62950.028.4458453
Produced by FastQC (version 0.11.2)