FastQCFastQC Report
Sun 14 Apr 2019
SRR6575868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087722
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22800.2096123825756949No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17530.16116250291894435No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12520.11510293990560087No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11630.10692070216470752No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11380.10462232077681614No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11090.10195619836686212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29200.043.5769581
ATCAACG37100.033.9529273
CAACGCA37900.032.988215
TCAACGC38100.032.9384084
AACGCAG39400.031.9709056
GTACATG65450.031.0344161
TATCAAC42450.030.448882
TACATGG67000.030.025162
ACATGGG65850.029.9784973
GGGTACC4850.029.070737
CTTATCG650.006152745328.9243093
CATGGGG34150.027.9396954
ACGCAGA45000.027.5719727
CGCAGAG45950.027.1029668
AGGTATA4800.026.4386276
GTCTTAG6650.026.2214091
ATACGGT909.3678304E-426.1820371
GCAGAGT48500.025.6779639
TGGGTAC5350.025.4777186
GGTATCA15250.025.3407781
Produced by FastQC (version 0.11.2)