FastQCFastQC Report
Sun 14 Apr 2019
SRR6575870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309898
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6230.2010338885697875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.17683237710472477No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5000.16134340976708467No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3260.1051959031681392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA200.002163708770.481734
TATAGGG608.80586E-539.1691552
GTATCAA15500.032.1439741
ATAGGGG753.2750398E-431.325213
GTACATG25050.030.397141
TACATGG24650.030.3183572
GTATAGG1102.9577623E-629.9109921
ACTTAGC650.00615511728.9155894
ACATGGG25800.028.7754843
CATGGGT4100.028.651114
CTATAAG1154.1813273E-628.6105141
AACGCAG17800.027.7175336
ATGGGTA2053.6379788E-1127.5050645
GTGTATA1057.401185E-526.8588491
ATGGGAG4200.026.850185
TATGGAC700.00883741126.850183
TAGGGGT1057.4174684E-526.850184
ATCAACG19000.025.9669513
GTATATG1101.01554695E-425.6379931
GTATAAT1659.266114E-825.6379931
Produced by FastQC (version 0.11.2)