FastQCFastQC Report
Sun 14 Apr 2019
SRR6575870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309898
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6190.1997431412916508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.18877178942748904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4850.15650310747407212No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4730.15263086563966208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT1001.4095549E-632.891644
TATACTT1452.6533598E-829.1650035
ATAAGTG650.006154962328.9157285
ATGGGAG4850.028.0959555
TACATGG26950.026.6759662
ACATGGG26750.026.1727873
GTACATG27600.026.0982651
CTAGAGT1901.328408E-824.7305564
GTATTAG1151.358415E-424.5630741
GTATCAA18450.024.2413351
GTCTTAG1751.6116428E-724.2121731
CATGGGA17600.022.9600754
CATGGGG7400.022.8590554
GAATAAG1252.3904741E-422.5980261
TATATAC2103.8424332E-822.3752653
ATATACT2103.8424332E-822.3752654
TATTATG1050.00233512322.3752652
CTACACA2103.8424332E-822.3752654
AACGCAG20950.021.7558086
ATGGGAT3251.8189894E-1221.6867965
Produced by FastQC (version 0.11.2)