FastQCFastQC Report
Sun 14 Apr 2019
SRR6575871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301359
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20990.16129292531883976No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17690.13593481890854098No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17120.13155478234676213No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16720.1284810724788471No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16120.1238705076769746No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16120.1238705076769746No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15580.11972099935528935No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15420.11849151540812336No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14890.11441884983313597No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14230.10934722855107622No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14200.10911670031098258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.045.2989851
GTATCAA21500.044.598941
TCAACGC26600.035.5138054
ATCAACG26700.035.3807953
CAACGCA27100.034.858575
TAGGTAT5050.034.4344445
AACGCAG28450.033.5348636
GTACATG50550.030.406011
ACCTAAG6350.030.3489021
GTCTTAG7350.030.0568431
TACATGG51600.029.9694672
ACATGGG51950.029.0403773
AATCGTC650.006155507628.9220716
GGTATAG6050.028.7428047
CTTAGGT6050.028.7428043
AGGTATA5950.028.4359866
TTAGGTA6150.028.275444
TATCAAC35150.026.8784082
ACGCAGA35900.026.5756787
CTAAGAC9050.025.9659483
Produced by FastQC (version 0.11.2)