FastQCFastQC Report
Sun 14 Apr 2019
SRR6575871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301359
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20120.15460760635612464No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19400.14907492859387764No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15300.11756940244774886No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15040.1155714910336041No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13530.10396823628222496No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13520.1038913935355271No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13320.10235453860156958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21500.056.3221551
TCAACGC26650.043.561054
ATCAACG27250.042.777673
CAACGCA28100.041.47895
AACGCAG29300.039.7801066
GGTATCA7850.039.614931
TATCAAC34400.034.1596572
ACGCAGA37850.030.7941117
CTTAGGT5850.030.5323123
TAGGTAT5600.029.3739195
CGCAGAG40300.029.1552568
AGGTATA5850.028.9220166
TACATGG53550.028.7025322
TTAGGTA5750.028.6087424
GTACATG53750.028.4898071
ACATGGG55300.027.624233
GTCTTAG7400.027.3792381
GCAGAGT44150.026.6118169
ACCTAAG9350.025.7005961
TCTTAGG8900.024.8221722
Produced by FastQC (version 0.11.2)