FastQCFastQC Report
Sun 14 Apr 2019
SRR6575872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155151
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17710.15331328977770006No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16030.1387697365972068No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15530.13444129815063138No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14670.12699638402252172No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14520.12569785248854912No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13580.11756038820898738No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13540.11721411313326137No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13070.11314538099348051No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12750.11037518038767226No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12400.10734527347506949No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11820.10232428487704204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19700.052.4924351
GGTATCA8050.043.209191
TCAACGC26500.038.6528634
ATCAACG27100.037.797083
CAACGCA28050.036.6844755
AACGCAG29350.035.059616
TAACGCT600.004166939831.3241844
TATCAAC33900.030.3657952
GTACATG49750.028.533441
ACGCAGA36450.028.2304387
TACATGG50400.028.0721872
ACATGGG51050.027.1517183
TGCGTAT700.00884862826.84930294
TTAGGTA3900.026.505084
CGCAGAG39200.026.2499878
GTCTTAG5650.025.7901291
TAGGTAT4050.025.523415
CTAAGAC8400.025.171223
AGGTATA4150.024.9083886
ATAGTAC1151.3799456E-424.514583
Produced by FastQC (version 0.11.2)