FastQCFastQC Report
Sun 14 Apr 2019
SRR6575872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155151
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18090.15660290299709734No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17780.1539192711602206No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14560.12604412756427513No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13920.12050372635265866No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12740.11028861161874075No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12610.10916321762263115No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12290.10639301701682291No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11940.10336311010422014No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11610.10050634072948038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19250.052.5580371
TCAACGC24850.039.901164
ATCAACG25000.039.473783
CAACGCA25950.038.0286945
AACGCAG26950.036.7919776
GGTATCA7550.036.150351
GTCTTAG7300.032.2313581
GTACATG45850.030.277091
TATCAAC33050.030.1448172
ACGCAGA32900.030.1381117
TAGGTAT5600.029.3703755
CTTAGGT6250.029.3233813
TACATGG46300.029.2320822
TTAGGTA5800.029.1678184
AGGTATA5600.028.5312216
ACATGGG48750.027.8581773
CGCAGAG35800.027.7159528
GGTATAG5900.027.080487
TCTTAGG8700.025.9280742
GCAGAGT38250.025.6947969