FastQCFastQC Report
Sun 14 Apr 2019
SRR6575873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454658
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44110.3032327873630778No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC39680.27277889373309744No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33580.23084463839610408No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17930.12325921281840818No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17610.12105938303023803No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17430.11982197877439234No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16650.11445989366572762No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16480.11329123409076222No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16320.11219131919667717No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16310.11212257451579682No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16290.1119850851540362No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16230.11157261706875432No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16070.11047270217466923No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15400.105866808555688No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15270.10497312770424388No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15190.10442317025720134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26750.048.6816371
TCAACGC30950.041.000854
ATCAACG31500.040.284963
GGTATCA10400.039.7794271
CAACGCA32700.039.094075
AACGCAG33250.038.5887536
TATCAAC39300.032.4179762
GTACATG62550.031.5668471
TACATGG62700.031.2663862
ACGCAGA42150.030.6637237
ACATGGG63700.030.103053
ACCAGAT17150.028.77596394
TAGGTAT10850.028.5893295
CGCAGAG46200.027.975678
GTCTTAG11300.027.4583641
CTAAGAC19000.027.210023
ACCGTAT700.00883758326.8566428
CTTAGGT11300.026.6189733
GGGTACC9500.026.2205667
TTAGGTA11150.026.1340434
Produced by FastQC (version 0.11.2)