FastQCFastQC Report
Sun 14 Apr 2019
SRR6575873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454658
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43160.29670204267944766No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34490.23710040435621296No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30460.20939629796144524No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16230.11157261706875432No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG16170.1111601489834724No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14850.10208585110727057No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14710.10112342557494614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26550.050.774591
GGTATCA9850.043.0676571
ATCAACG32200.041.01473
TCAACGC32550.040.861064
CAACGCA34100.039.6928525
AACGCAG34750.038.9503946
GGTAATC3450.038.1415378
TATCAAC37350.035.3593982
GTACATG62400.031.95221
TAGGTAT9600.031.3316255
ACGCAGA42750.031.330557
GTCTTAG11000.031.2806051
TACATGG63950.031.014232
TTAGGTA9750.030.3675774
ACATGGG65800.029.927973
CTTAGGT10350.029.5163143
CGCAGAG45450.029.3659278
AGGTATA10400.028.9215016
ACCTAAG19050.027.7120081
GCAGAGT49200.027.127679
Produced by FastQC (version 0.11.2)