FastQCFastQC Report
Sun 14 Apr 2019
SRR6575874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327906
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43420.3269809760630647No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC38170.2874450450559001No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31880.2400772343825542No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17760.1337444066070942No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16470.12402986355961942No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16310.12282495899559155No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16280.12259903938983632No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16060.120942295614298No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16010.12056576293803928No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15870.1195114714445149No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15240.11476715972365513No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15110.11378817476538249No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15020.1131104159481168No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14210.10701058659272569No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14120.10633282777546No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13810.103998325182656No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13430.1011366768430898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22000.056.1946531
TAACTCG451.6170003E-552.206324
TCAACGC27300.044.404064
ATCAACG27800.043.2674033
GGTATCA7100.043.0345151
CAACGCA28600.042.3856965
AACGCAG29450.041.321886
TATCAAC35400.034.259292
GTACATG56400.032.921521
ACGCAGA37900.032.108957
TACATGG57300.031.912212
GTCTTAG13050.031.6981621
ACATGGG58300.031.189473
CTTAGGT11600.029.9736293
TAGGTAT11450.029.9559445
CGCAGAG40950.029.8321848
AGGTATA11700.029.315866
TTAGGTA12100.029.1233634
TCTTAGG14200.027.8069172
GCAGAGT45650.026.9665959
Produced by FastQC (version 0.11.2)