FastQCFastQC Report
Sun 14 Apr 2019
SRR6575874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327906
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42880.3229144231594706No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34350.25867794858973453No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31490.23714027950773622No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG16400.12350271781285724No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16320.12290026553084331No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT15630.11770411459847308No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14270.10746242580423615No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13940.10497731014092865No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13880.1045254709294182No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13610.10249219447762115No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13540.10196504873085895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26250.049.1446651
GGTATCA10650.044.650551
TCAACGC31050.040.559584
ATCAACG31400.040.2571373
CAACGCA31700.039.8761525
AACGCAG32700.038.8004046
TATCAAC39000.032.53512
TTAACGC600.004164852731.3277343
GTACATG61750.030.4984721
TACATGG62850.029.7598532
AGGTATA10200.029.484936
ACGCAGA43050.029.4731947
TAGGTAT10150.029.16725
GTCTTAG11150.029.1359581
ACATGGG64200.029.1318653
CTTAGGT10500.028.6425023
TTAGGTA10400.028.0142234
CGCAGAG46050.027.6739238
ACCTAAG17550.027.0956341
GGTATAG11350.026.9125027