FastQCFastQC Report
Sun 14 Apr 2019
SRR6575875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666636
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21960.32941515309704245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19320.28981333141324506No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC15500.23251069549199266No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15450.23176066099040554No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA14350.21525990195548997No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11930.17895823207867562No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11800.17700814237454923No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10220.15330705212439774No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT10220.15330705212439774No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9370.14055646559741747No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9240.13860637589329108No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8980.13470619648503832No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT8310.12465573416377154No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7720.11580532704504407No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7700.11550531324440924No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7660.11490528564313959No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA7320.10980505103234749No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG7140.10710492682663403No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7040.10560485782345988No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT6790.10185468531552452No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG6770.10155467151488969No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6720.10080463701330261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACTG1900.044.52454
GTGTAAT500.00169872237.6125761
ACCTAAG1451.8189894E-1135.66711
GTACTAG4700.033.0110361
CCTAAGA1658.54925E-1131.3438152
AATCGAC1102.9669209E-629.9078736
ATGGGGG11700.029.7252855
CATGGGG17850.029.4889954
TATTAAG804.7694054E-429.3848252
ATGGGCG650.00615356328.92195
GAACCGT1005.3217023E-528.1988536
TAGTACT4850.028.1019524
TAGGCAT6450.027.6887975
GTACTGT5450.027.595216
TACCCCG700.00883516126.856055
TCAAGAC2305.456968E-1226.5641353
TGGGGGG6400.026.4364246
CTAGGCA7100.025.8158514
CATGGGT4200.025.7370474
ATGGGTA2208.731149E-1125.635325
Produced by FastQC (version 0.11.2)