FastQCFastQC Report
Sun 14 Apr 2019
SRR6575876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637083
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23270.3652585298932792No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20910.32821469102142103No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14520.2279137883132967No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13680.21472869312161835No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13530.2123742118373901No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10970.17219106458656094No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10650.1671681711802073No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9020.1415828078915934No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC8850.13891439576946804No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8780.13781563783682818No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8740.13718777616103395No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8720.13687384532313687No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8390.13169398649783465No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8240.12933950521360638No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7630.11976461465774475No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7610.11945068381984765No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT7010.11003275868293456No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6900.10830613907450048No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6820.10705041572291209No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6760.10610862320922078No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG6590.10344021108709539No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA6550.1028123494113012No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6550.1028123494113012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG801.1590655E-535.2445562
GCTTAAG702.1698198E-433.608451
GTCCTAC3050.029.3109761
GTGTATA1652.924935E-928.5162621
ATACCGA1205.8448386E-627.412436
CATGGGG18700.026.3862974
TACACTA4300.026.2285065
ATGGGGG12050.026.1287425
GTACTAG4150.026.0769181
TAGGACT3450.025.880064
CGTTAAC2008.0035534E-1025.8785081
ACCTAAG2008.0035534E-1025.8785081
TAGGTAT1101.0187071E-425.6324045
GTAGGAC3350.025.249833
GACTCGT3350.025.249837
TAACAGT2051.0822987E-925.2156164
CTACACT4350.024.8467354
GTATAAG3600.024.8329121
TAACCCT950.001305121924.7330194
CACTATC4200.024.6152447
Produced by FastQC (version 0.11.2)