FastQCFastQC Report
Sun 14 Apr 2019
SRR6575877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820757
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22010.26816707015596575No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21820.2658521340664776No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC19750.24063151456521237No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC15840.1929925666183779No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14350.17483859412712896No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13730.1672845921509046No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12420.15132371700759178No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11410.13901800411083914No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA11080.13499732563962294No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10200.12427551638304639No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9920.12086403161959021No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9550.11635599818216598No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9190.11196980348629375No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8910.10855831872283758No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8590.10465947899317338No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT8450.10295373661144529No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG8340.10161351045437322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC857.927156E-944.2341773
CTTAGGT1755.456968E-1232.2277573
GTACTAG4750.030.6841241
GCATATA1551.5133992E-930.3329011
TAGTACT7500.028.199294
TAATAAT4000.028.1992863
GTACTGT7850.028.1394186
ACCTAAG2750.027.3547631
CTTTATA1057.401868E-526.8662832
TTATATC700.00883566726.8564644
ACCGTGC700.00883566726.8564648
CTAGTAC8950.026.2563193
GTATACA909.4964664E-426.1199991
CTAACTG2700.026.110454
CATGGGG25600.025.8860654
CCTAGTA8650.025.5462632
CTGTGCG8400.025.1779359
ATGGGGG16700.025.0472725
TAGGTAT1901.3320459E-824.7362165
GACGTTA1351.4570671E-524.3697537
Produced by FastQC (version 0.11.2)