FastQCFastQC Report
Sun 14 Apr 2019
SRR6575878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761757
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25300.3321269118629694No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24490.32149359966498503No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16620.21817981324753166No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13390.17577783991482848No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12140.15936840751053158No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11280.1480787180163753No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10360.1360013757668128No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10270.1348198966337034No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10020.13153801015284403No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9990.1311441837751409No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9370.12300510530260964No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8550.11224051764539085No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8500.11158414034921897No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8430.11066521213457835No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8410.1104026612161096No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8280.10869608024606271No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT8020.10528291830596898No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7940.10423271463209395No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7740.10160720544740647No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7640.10029445085506271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTG608.7942346E-539.187088
CTTAATA801.1581698E-535.249852
ACCTAAG2200.032.106451
CCTAGTT753.2745916E-431.3332022
TACATAA753.2745916E-431.3332022
TAATAGA600.004162868531.3290884
TATAGTG600.004162868531.3290885
TGGGACG600.004164206331.3270326
GTACTAG4400.028.8958031
TAGTAGT2002.7284841E-1128.196184
AACCGTG1258.031806E-626.3147057
GTAGGAC4350.025.927523
GTAGTGT1659.327232E-825.6312086
ATACGGG5000.025.376563
TAAGGTG4650.025.2653944
CTATTAG5400.025.2406372
GTATAAG5050.025.176541
TATTAGG5450.025.0057873
ATAAGGT4900.024.9353963
TACGGGC5000.024.4366874
Produced by FastQC (version 0.11.2)