FastQCFastQC Report
Sun 14 Apr 2019
SRR6575881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325625
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6760.2076007677543186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.16552783109404992No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5000.15355086372360846No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4480.13758157389635317No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4350.13358925143953934No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4040.12406909788867562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9950.048.2758561
ATCAACG13600.036.63693
AACGCAG13750.036.579096
TCAACGC13900.035.8461764
CAACGCA14000.035.5901345
TCCTACG550.002714553634.18081394
ACATGGG21900.032.1957783
TATCAAC15350.032.1538282
TACATGG22100.032.117112
GTACATG22450.031.884541
ACGCAGA16750.030.0237
CGCAGAG17350.029.2556238
GTATTAC650.00608891228.980081
CATGGGG8950.028.3610634
CATGGGT5450.027.5997564
GCAGAGT19050.026.3981729
GTATAGG1257.879396E-626.3718721
GCTATAC909.496096E-426.1143533
CTAACCG459.514866E-426.11034450-51
CATGGGA8500.025.991464
Produced by FastQC (version 0.11.2)