FastQCFastQC Report
Sun 14 Apr 2019
SRR6575884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589982
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7270.12322409836232293No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7080.12000366112864459No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7060.11966466773562583No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6370.10796939567647827No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6170.10457946174629057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.057.2751921
TCAACGC10650.039.7174874
GGTATCA3150.037.3039741
ATCAACG11400.037.1044963
CAACGCA11500.036.3731585
GTCTTAG3900.034.9509541
AACGCAG12200.034.6714136
TTAGGTA2350.031.9993364
CTTAGGT2750.030.7629973
AGGTATA2450.030.693246
TATCAAC13800.029.9729332
TCTTAGG3850.029.300582
ATTATCC4200.029.0946353
ACGCAGA14700.028.4551937
TAGGTAT2650.028.3767685
CGCAGAG15550.027.202018
GTCTAAT700.00882984126.8588641
TCTAGAC1057.4166026E-526.8565853
TAAGACC1057.4166026E-526.8565854
GGTATAG3000.026.6327827
Produced by FastQC (version 0.11.2)