FastQCFastQC Report
Sun 14 Apr 2019
SRR6575884_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575884_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589982
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7000.049.814671
GGTATCA3350.037.9789121
TCAACGC9700.035.8573234
CAACGCA9750.035.6734355
ATCAACG9550.035.4361843
AACGCAG10250.033.933276
TAGGTAT3100.033.3563735
TTAGGTA3000.032.9015124
GTCTTAG4250.029.936321
GGTATAG3350.029.4615447
GTATAGT3700.027.9448628
TATCAAC12150.027.853132
CTTAGGT3550.027.8040943
AGGTATA3400.027.648336
GTCCGTA700.00872163726.926851
TATAGTA3900.026.5117919
TACATGG17700.026.0237942
ACGCAGA13050.025.9300297
GTACATG18000.025.917091
TATAGTG1301.0862708E-525.3088555
Produced by FastQC (version 0.11.2)