FastQCFastQC Report
Sun 14 Apr 2019
SRR6575885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443350
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5900.13307770384572007No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5690.12834103981053344No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5570.12563437464756963No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5540.12495770835682868No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5320.11999548889139505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT5010.11300327055373859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4970.11210104883275064No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4740.10691327393707002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5550.053.3370171
ATAGACC704.6373516E-640.2748873
ATCAACG7450.038.472883
TCAACGC7900.037.4709364
CAACGCA7800.037.3489345
AACGCAG8500.034.825936
GTACATG15800.031.5231691
ACGCAAC753.277483E-431.3249136
GTCTTAA1551.5188562E-930.3144321
TACATGG16500.030.1858252
ACATGGG17200.029.5036963
CAACCGA804.783723E-429.3671059
TAGGCTG804.783723E-429.3671055
TATCAAC10100.028.3785112
TTAGGTA1503.696732E-828.1924214
GTCTTAG2350.027.9924761
TACGCAA856.8201456E-427.639635
TAGTGAG1205.844775E-627.4092985
CTAAGAC3100.027.2829883
ACGCAGA10550.027.168057