FastQCFastQC Report
Sun 14 Apr 2019
SRR6575885_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575885_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443350
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4640.10465771963460022No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT4600.10375549791361227No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG4470.10082327732040149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6000.049.370741
TTAGGTA1850.038.1026234
ATCAACG7700.037.2284053
GGTATCA2800.036.9440841
CAACGCA8000.035.8323445
TCAACGC8000.035.8323444
TAGGTAT2100.035.8043675
TATCAAC8700.034.0295832
AACGCAG8600.033.332416
TATGTAC1001.4101588E-632.8952643
CTTAGGT2300.032.6909453
GTACATG14850.030.0799731
TACATGG14650.029.831882
ACCTAAG2101.8189894E-1229.1074641
TCTTATA650.00613753528.935231
TTAACGG650.006154474328.9189154
GTCTTAG2450.028.7876011
GGTATAG2300.028.6045787
AGGTATA2500.028.195946
ACATGGG16000.027.9022333
Produced by FastQC (version 0.11.2)