FastQCFastQC Report
Sun 14 Apr 2019
SRR6575888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268669
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20260.15969492436561467No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15190.11973178189110005No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14760.1163424029435574No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12910.10176019119250174No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12790.10081431799783867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22200.048.6966131
GGTATCA9100.044.4202461
TCAACGC28450.037.1697734
ATCAACG28750.036.945393
CAACGCA28900.036.5910075
AACGCAG30300.035.2105566
GTACATG57650.030.6557851
TATCAAC35800.030.066052
TACATGG59600.029.337332
ACATGGG59450.029.250923
GGGTACC8800.027.7722037
GGTAATC2053.6379788E-1127.5116758
GGTACCT9250.027.437328
ACGCAGA38700.027.3250687
GTCTTAG7300.026.3988421
TAGGTAT4200.025.737615
CGCAGAG41900.025.5746968
TGGGTAC9800.025.4178896
CATGGGG30800.025.0255014
ACCTAAG5750.024.5232091
Produced by FastQC (version 0.11.2)