FastQCFastQC Report
Sun 14 Apr 2019
SRR6575889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139328
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18710.1642196101561622No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14990.1315687844062465No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13090.11489228738344005No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12010.10541301539152903No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11910.10453530502190765No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11420.10023452421076284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21800.051.968331
GGTATCA8250.045.0143851
TCAACGC28600.038.609074
CAACGCA29600.037.304715
ATCAACG30100.036.685033
AACGCAG30950.035.677536
TATCAAC34450.032.3412172
TAGGTAT5300.031.9164225
AGGTATA4950.031.3253756
TACTCTA753.280406E-431.3253755
GTATACG804.7756816E-429.3804341
GTACGGT650.00614858628.9284271
GTCTTAG7850.028.744171
CTTAGGT5300.028.3701523
ACGCAGA39050.028.2770677
GTACATG52800.027.9559271
CGCAGAG41250.026.8828668
ACATGGG53350.026.8629043
TTAGGTA5600.026.8503234
GGTATAG6000.026.6265687
Produced by FastQC (version 0.11.2)