FastQCFastQC Report
Sun 14 Apr 2019
SRR6575889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139328
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18220.15991882934501742No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15650.13736167284574766No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13310.11682325019660714No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12190.10699289405684755No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11900.10444753398494552No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11520.10111223458038424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.053.292261
GGTATCA7900.049.454251
TCAACGC25600.040.934794
ATCAACG26200.039.8179933
CAACGCA26900.038.9582375
GTCTTAG7050.038.0573351
AACGCAG27750.037.7616126
TAGGTAT5000.034.775915
CTTAGGT5550.034.715113
AGGTATA4950.034.1747976
TATCAAC31200.033.891732
TTAGGTA5850.032.934854
TCTTAGG8400.031.8905052
GTACATG51100.031.503431
GGTATAG5700.030.5025027
TACATGG52750.030.4697912
ACATGGG52200.030.3380093
ACGCAGA35350.029.6431317
CTAAGAC9100.028.4020043
CGCAGAG37350.028.1989548
Produced by FastQC (version 0.11.2)