FastQCFastQC Report
Sun 14 Apr 2019
SRR6575890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765813
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33950.19226271411525456No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA33310.18863832127184477No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28350.16054927673541877No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22210.12577775789395593No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22040.12481502854492521No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21950.1243053483013207No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20910.11841570993077975No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20610.11671677578543142No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20350.11524436619279618No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19620.11111029310578185No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18950.10731600684783724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29650.046.768791
TCAACGC36250.037.9878434
ATCAACG37150.036.9410363
CAACGCA37800.036.430145
TAGGTAT10000.035.2489625
AACGCAG39450.035.025586
GGTATCA13150.034.3166051
AGGTATA10800.032.6379246
TTAGGTA11250.032.1679424
CTTAGGT11300.032.02563
GTCTTAG12350.031.97211
TATCAAC46800.029.3289032
TCTTAGG14750.029.3193682
GTACATG72150.028.9922891
GGTATAG12450.028.6899157
TACATGG72800.028.539722
GTATAGT12550.028.4613138
ACGCAGA50150.027.646247
TATAGTA13100.027.266379
ACATGGG74900.027.1073443