FastQCFastQC Report
Sun 14 Apr 2019
SRR6575891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551384
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA33410.21535609494490082No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31630.20388246881494201No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27530.1774544535717785No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19860.12801472749493356No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19110.12318033446264755No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18030.11621880849615569No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17400.11215791834903543No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16590.10693677387416656No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16170.10422951377608639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.043.5507661
ATCAACG29350.036.8269273
TCAACGC29500.036.6396684
CAACGCA31550.034.4079175
TTAGGTA8800.033.643694
GTCTTAG10400.033.051411
AACGCAG33500.032.5453456
GGTATCA11900.032.4464651
AGGTATA9650.031.1672446
CTTAGGT9750.030.8475763
TAGGTAT9800.030.6901935
GTACATG60750.029.8411061
CTAAGAC14300.028.9196053
TACATGG62600.028.6779942
TATCAAC38450.028.6008682
ACCTAAG13200.028.5375391
TAAGACA15550.028.4081654
ACATGGG63900.027.8730243
GGTATAG10700.027.6686827
TCTTAGG12550.027.3362452