FastQCFastQC Report
Sun 14 Apr 2019
SRR6575892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293258
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6290.21448690231809533No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3920.1336706927006254No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3820.13026072605009922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.11593886611788937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9450.045.7585261
ATCAACG11850.036.478533
GGGTACC3100.036.3762057
AACGCAG11900.036.325266
TCAACGC11900.035.930424
CAACGCA12150.035.1911135
CGAGCGT352.1875446E-433.56138264-65
GGTACCT3400.033.1665428
GTACATG17950.031.9456211
ACGCAGA13600.031.7846057
CGCAGAG13750.031.7795758
TGGGTAC3700.031.7472526
CATGGGT5350.031.6167014
GACATAT600.00415512431.3346421
TACATGG18450.031.0798872
ACATGGG18650.030.988043
ATGGGTA3850.030.5103455
TATCAAC14450.030.2503992
ATAAGAC804.7806735E-429.3662093
CGCCGGT804.7806735E-429.3662097
Produced by FastQC (version 0.11.2)