FastQCFastQC Report
Sun 14 Apr 2019
SRR6575893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813676
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15580.1914767057157886No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15210.18692944120264085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14020.17230445533603056No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12610.1549756905697108No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12410.1525177097517931No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12150.1493223346885001No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10710.13162487279949267No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10230.1257257188364902No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9780.12019526199617539No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9290.11417320899227704No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9150.11245262241973462No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8910.10950304543823339No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8820.10839695407017043No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8590.10557027612956509No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8230.10114591065731324No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8160.10028561737104202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA952.111301E-839.5881351
GTACTAG5150.037.426161
TAGTACT6000.030.5477054
GAACCGT1252.363322E-730.0777386
CATGGGG22100.029.3462124
TAGGCAT6750.029.2422455
TCTTAGG1952.0008883E-1128.929792
CATACGA650.00615538828.9209025
GTACTGT6900.028.6065466
CTGTGCG7450.027.7563029
TATTAAG856.8051234E-427.6534772
ATGGGTA4600.027.5848835
TGGGTAC4000.027.0229666
CGTTAAC1755.5351848E-926.8633771
AACGTAG350.00884032126.85512592-93
TAATAAT2800.026.8551253
ACTATCG700.0088377726.8551255
TAGGTAT1406.3686457E-726.8551255
CGGGCGT5850.026.510836
TACTGTG7850.025.7432867
Produced by FastQC (version 0.11.2)