FastQCFastQC Report
Sun 14 Apr 2019
SRR6575893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813676
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16420.20180022515104293No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15950.19602397022893633No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15190.18668364312084906No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12850.15792526755121203No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12410.1525177097517931No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10950.13457444978099392No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10880.1337141564947227No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10380.12756920444992847No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10230.1257257188364902No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9910.12179294952782188No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9760.11994946391438362No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9690.11908917062811242No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9410.11564799748302763No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9360.11503350227854822No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8460.10397258859791858No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8450.1038496895570227No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8330.10237490106627208No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8200.10077721353462558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT1003.3196557E-837.6067354
CGTTAAC1703.6379788E-1233.266251
AAGGGTA1001.4085199E-632.9058955
GTCCTAC4150.031.7966561
GGGTACC4000.031.7228857
TACATAA600.00415537631.3408742
GTATAAG6100.030.9030741
ACGTTAA1401.8908395E-830.212278
TGGGTAC4100.029.8102196
TAGTACT6650.029.6895284
TATAGTG804.772939E-429.3820692
ACGCAAG650.006155130528.9211487
ATGGGTA4500.028.2050535
GTACTAG4750.027.7802371
CATGGGG20250.027.3925614
ATGGGGG12700.027.020595
CACTATC5050.026.98837
CTTAGGT1755.5351848E-926.8636053
GAACCTA700.00883740626.855359
TACACTA4750.026.7205755
Produced by FastQC (version 0.11.2)