FastQCFastQC Report
Sun 14 Apr 2019
SRR6575894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776117
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18600.23965458816132104No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16140.2079583361786947No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14050.18102940664873982No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12720.1638928151296776No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12220.15745048749093243No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12060.15538894264653394No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10430.13438695454422464No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9640.12420807687500725No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9450.12175999237228408No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9420.12137345271395937No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8860.11415804575856474No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8800.11338496644191533No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8510.10964841641144313No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8250.10629840603929562No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8180.1053964801698713No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7980.10281954911437322No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7890.10165993013939909No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7770.10011377150610024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG550.00271931734.174515
AGGTATA851.756619E-533.1693766
GTACTAG4550.032.013071
GACGTTA1252.3659595E-730.0735667
TATTACG1154.2010706E-628.6025775
ACGTTAA1354.6400964E-727.8458948
CTAACTG2750.027.3396074
GTATAAG3800.027.2029551
TAGGCAT5900.026.2825135
CTAACGC3250.026.0252043
TCAAGAC2357.2759576E-1225.994443
TAGGTAT1101.0193996E-425.6308825
CCTATTC5150.025.5479323
TAGTACT5150.025.5479324
GGGTACC3500.025.508837
CATGGGG21100.025.3879364
CATGGGT6000.025.0613064
TCTAACG3600.024.8002512
TGGGTAC3650.024.4605226
GTCCTAT4050.024.363591
Produced by FastQC (version 0.11.2)