FastQCFastQC Report
Sun 14 Apr 2019
SRR6575895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697062
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13270.19037044050600951No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11670.16741695860626457No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10330.14819341751522822No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10050.14417655818277283No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9880.14173775073092493No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9000.12911333568606523No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8740.1253833948773567No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8150.11691929842682573No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7940.11390665392748422No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7620.10931595754753523No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7520.10788136492880117No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7220.10357758707259898No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7110.10199953519199154No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7070.10142569814449792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG2250.031.3377341
AACGCAA650.0061526228.922997
TAGTACT3300.028.4847624
ACGCCTA2203.6379788E-1227.7726426
CTTAGGT1904.474714E-1027.2104433
CGTACAC700.00883381226.857063
CTAACGC2305.456968E-1226.5651363
TTAGGTA2008.112693E-1025.8499224
TCTAACG2550.025.8075452
CATGGGG20650.024.8086414
TAATAAT1901.33095455E-824.7367673
GGGTACC3900.024.1024917
GTACATG43850.024.0124911
CGCCTAC2552.1827873E-1123.960717
ACATGGG43300.023.771293
TACATGG44100.023.7697772
CGTTAAC1000.001756551623.5033021
TCTTAGG3000.023.50332
GTAGACC1000.001758018723.499933
AGGTATA2202.4465407E-923.4999286
Produced by FastQC (version 0.11.2)