FastQCFastQC Report
Sun 14 Apr 2019
SRR6575895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697062
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12020.17243803277183378No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11570.16598236598753052No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9850.1413073729453047No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8950.1283960393766982No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8560.12280112816363538No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7850.11261552057062357No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7840.11247206130875016No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7720.1107505501662693No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7590.10888557976191501No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7320.10501217969133304No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7120.10214299445386495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGT1256.2536856E-933.842943
TAGGTAT1256.2591425E-933.8405155
TTAGGTA1351.3244062E-831.3360584
GTATAAG2002.5465852E-1128.2693791
TATTAAG1503.6903657E-828.2024522
GTCTTAG3350.028.1287361
ACCTAAG2750.027.4127331
GTACTAG2750.027.4127331
CTAACGC1458.635725E-725.933293
TCTTAGG2008.094503E-1025.852252
AGGTATA1659.305404E-825.6367556
GTATTAC1301.066503E-525.3699571
CATGGGG19450.024.8916284
GTATTAT950.001285526224.79771
GTCCTAA950.001285526224.79771
TACATGG42950.023.5294062
TAGGCAT3200.023.5003595
ACATGGG41900.023.4459513
GTACATG42850.023.2004641
TGAACCG3050.023.1151075
Produced by FastQC (version 0.11.2)