FastQCFastQC Report
Sun 14 Apr 2019
SRR6575896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658828
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14860.2255520408968653No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13780.2091592949904983No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9970.1513293302652589No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9760.14814185189457643No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9380.1423740338904843No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8880.13478479967457363No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8370.12704378077434475No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7510.11399029792297838No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7480.11353494387002373No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7310.1109546042366141No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7280.11049925018365946No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7270.11034746549934125No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7220.10958854207775018No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6710.1018475231775213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2250.039.689691
AGGTATA1551.8189894E-1236.3767856
TTAGGTA1700.035.9309924
TAGGTAT1601.8189894E-1235.2400135
CTTAGGT1703.6379788E-1233.167073
CTAACTG1807.2759576E-1231.3244554
GTATAAG2500.028.200571
ATTTAGT700.00883246426.8576831
GGGTACC3850.026.8495317
CTAACGC1406.3771586E-726.8495313
CTAGGCA3950.026.1697964
GTCTTAG3800.025.974211
ATGGGTA4350.025.9236855
TCTAACG1501.1610664E-625.0671732
TAGGCAT3750.025.0595635
ACCTAAG3200.024.9692551
GGTACCT4150.024.90868
TGGGTAC4150.024.90866
GGTAATC950.001306150124.7298328
CTATCCT950.001306150124.7298324
Produced by FastQC (version 0.11.2)